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Journal of Stem Cell Research and Tissue Engineering

JSCRTE
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Published by Universitas Airlangga

ISSN : 26141264 EISSN : 26141256 DOI : https://dx.doi.org/10.20473/jscrte

Core Subject : Health, Science, Engineering,

Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary

Journal of Stem Cell Research and Tissue Engineering (JSCRTE) is published by Stem Cell Research and Development Center, Airlangga University. Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancerstem cells, developmental studies, genomics and translational research. Special focus of JSCRTE is on mechanisms of pluripotency and description of newly generated pluripotent stem cell lines. Articles that go through the selection process will be review by peer reviewer or editor. The journal is published regularly twice a year in December and May. Every publication consists of 60-70 pages and 5 scientific articles in the form of research, study literature, and the case study in English. The contributors Journal of Stem Cell Research and Tissue Engineering are Stem Cell researchers, lecturers, student and practitioners that came from Indonesia and abroad.

Arjuna Subject : Kedokteran - Imonologi dan Alergi

Articles 5 Documents

Search results for , issue "Vol. 3 No. 1 (2019): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING" : 5 Documents clear

Escalating Dose Antigen Specific Therapy with dsDNA Injection Regulate Balance Ratio of Inflammatory Cells in Pristane-Induced Lupus Mice Model sri poeranto
Journal of Stem Cell Research and Tissue Engineering Vol. 3 No. 1 (2019): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Immunosuppressant and steroid therapy for SLE have not shown satisfactory results. Another method of therapy that is being developed is vaccines and escalating dose immunotherapy using self-antigen. The aim of this study was to assess the balance of immune cells through the ratio of pro-inflammatory and anti-inflammatory cells and cytokines in SLE using self-antigen dsDNA therapy. Methods: Female Balb/c mice 6-8 weeks old separated randomly to negative control group and pristane induced lupus (PIL) mice group. PIL mice groups were injected pristane intraperitoneally. Twelve weeks after the injection, the mice were evaluated for clinical and serological manifestations (anti-dsDNA levels). Mice with lupus signs were divided into four groups; positive control group: PIL mice without EDI dsDNA therapy, treatment A: PIL mice with EDI dsDNA therapy dose I (0.01μg/ml, 0.1μg/ml, 1μg/ml), treatment B: PIL mice with EDI dsDNA therapy dose II (0.1μg/ml, 1μg/ml, 10μg/ml), and treatment C: PIL mice with EDI dsDNA therapy dose III (1μg/ml, 10μg/ml, 100μg/ml). dsDNA were injected once a week and the dose was increased every week. Samples were analyzed for active/inactive dendritic cells ratio, Th1/Th2 cells ratio, Th17/Treg cells ratio and IL-17/TGF-β levels ratio. Results: Escalating dose antigen specific therapy with dsDNA injection of third dose reduced active/inactive dendritic cells ratio (p=0.000), Th1/Th2 cells ratio (p=0.010), Th17/Treg ratio (p=0.004) and decrease IL-17/TGF- β levels ratio (p=0.004) significantly compared to positive control. Conclusion: Escalating dose antigen specific therapy with dsDNA injection of dose III was able to regulate balance ratio of inflammatory cells and cytokines in PIL mice thus the immune tolerance may improve compared to control groups.

3D Differentiation Of Mammosphere Derived Macaca fascicularis’s Mammary Stem Cells silmi mariya
Journal of Stem Cell Research and Tissue Engineering Vol. 3 No. 1 (2019): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

The mammary gland contains adult stem cells that are capable of self-renewal. This population plays an important role in the development of mammary gland and breast cancer pathogenesis. The studies of mammary stem cells are limited due to the difficulty to acquire and expand adult stem cell population in an undifferentiated state. In this study, we developed mammosphere cultures of nulliparous cynomolgus monkeys (Macaca fascicularis; Mf) as a culture system to enrich mammary stem cells. This species has similarity of mammary gland structure as humans including anatomy, developmental stages, and lobule profile of mammary gland. The use of stem cells from primate animals is essential to bridge the knowledge gaps resulting from stem cell research using rodents for clinical trials in human. Small samples of mammary tissues were collected by surgical biopsy; cells were cultured as monolayer and cryopreserved. Cryopreserved cells were cultured into mammospheres, and the expression of markers for mammary stem cells was evaluated using qPCR. Cells were further differentiated with 3D approaches to evaluate morphology and organoid budding. The study showed that mammosphere culture resulted in an increase in the expression of mammary stem cell markers with each passage. The 3D differentiation in matrigel allowed for organoid formation. Mammary gland stem cells have been successfully differentiated which characterized by CSN2 marker expression and differentiation regulators marker STAT5 and GATA3. The results indicate that mammospheres can be successfully developed derived from breast tissue of nulliparous Mf collected via surgical biopsy. As the mammosphere allows for enrichment of mammary stem cell population, the findings also suggest that a 3-dimensional system is efficient as in-vitro model to study mammary stem cells and a useful system to study mammary differentiation in regards to cancer prevention.

Histomorphology of Pancreatic Islet in Physiological Aging Female Rats Post Intravenous Human Wharton’s Jelly Mesenchymal Stem Cell Injection wining astini
Journal of Stem Cell Research and Tissue Engineering Vol. 3 No. 1 (2019): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

The increasing population of aged people will have the important role in the life, but the function of their bodies will decrease because of aging. Aging will increase the risk of degenerative disease, one of example is diabetes. The disease is related to the aging in the pancreatic organ which progressively declines by age. The aimed of the experiment was to determine the effect of human wharton’s jelly mesenchymal stem cells by injecting intravenously in aging female rats. This study used 3 young female rats (3 months) and 6 aging female rats (24 months). The experiment consisted of three groups. The young control group (A), the aging control group (B) that received NaCl (0.9%) 0,4 mL, the aging treatment group (C) received 1 x 106 cells/kg of human wharton’s jelly mesenchymal stem cells 0,4 mL. The aging control and the aging treatment group were injected 4 times with the interval in 3 months. The end of the experiment (12 months), the rats were anesthetized and sacrificed. The pancreatic tissues were collected to examine the pancreatic islets by histology studies. Changes of the pancreatic islet in control and treated groups were examined using hematoxylin and eosin staining. These findings conclude that injecting human wharton’s jelly mesenchymal stem cell increase the diameter and total pancreatic islet in the treatment group. In other side, the cell population of pancreatic islet also have significant differences (P<0.05) in treated physiological aging female rat groups than control aging female rat group.

Desensitization with Self-Antigen dsDNA Inhibits B and T-cell Functions by Modulating Treg as Regulator Immune System in Pristane-induced Lupus Mice Model saiful arifin
Journal of Stem Cell Research and Tissue Engineering Vol. 3 No. 1 (2019): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

The aim of this study was to develop a novel therapeutic method for improving immune system regulation in SLE using escalating dose self-antigen dsDNA immunotherapy. Methods: Female Balb/c mice were given a single intraperitoneal injection of 0.5 ml pristane.. Starting at 12 weeks after injection,. the mice were evaluated for clinical and serological manifestations. Mice with lupus signs (PIL mice) were divided into four groups; positive control group, PIL A (0.01 μg/ml, 0.1 μg/ml, 1 μg/ml ) EDI dsDNA, PIL B (0.1 μg/ml, 1 μg/ml, 10 μg/ml ) EDI dsDNA, and PIL C(1 μg/ml, 10 μg/ml, 100 μg/ml). EDI dsDNA were administered once every week in consecutively. The doses would increase every week. dsDNA were complexed with the cationic polyethylenimine (PEI) before injection. Samples were analyzed for autoantibodies levels (dsDNA, ANA) and ,TGF-β cytokine from serum using ELISA and T-Reg, mature dendritic cells from spleen using flowcytometry.. Results: Escalating dose antigen spesific immunotherapy with self-antigen dsDNA significantly decreased ANA (p=0.02), anti-dsDNA (p=0.03), dendritic cell mature (p=0.02) compare to positive control, and not significantly decreases Th17 cells (p=0,18) but the result tend to get lower. Desensitization using self-antigen dsDNA was increased T-reg proliferation (p=0.00) and level of TGF-β (p=0.03) significantly compare to positive control. Conclusion: Desensitization using self-antigen dsDNA coupled to PEI was able to modulate T-Reg as a regulator immune respon and inhibit B and T cell functions in lupus mice model.

Multiple Injection of Human Wharton’s Jelly Mesenchymal Stem Cells Ameliorate Spermatogenic Cells on Physiological Aging Male Rats alif imam fitrianto
Journal of Stem Cell Research and Tissue Engineering Vol. 3 No. 1 (2019): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Recently, the most common therapy on men who suffered fertility decline due to aging was testosterone replacement, but now it is known that this therapy has a long-term risk of damage to the cardiovascular system and prostate. Stem cells are an alternative therapy that has a potency to improve the fertility of aging men that less causing side effects. The aim of this study was to evaluate the injection of Human Wharton’s Jelly Mesenchymal Stem Cells (hWJ-MSC) in physiologic aging male rats on spermatogenic cells. This study used 3 young male rats (8-12 weeks) and 6 physiological aging rats (22-24 months) which divided into 3 groups, the young rat group did not give any treatment, physiological aging male rats received NaCl (0.9%) 0.4 mL, and physiological aging male rats received 1x106 cells/kg BW of hWJ-MSCs. The observations were performed on histological analysis. The results indicate that the hWJ-MSCs injections increased the number of spermatogonia and Leydig cells significantly (P<0.05), and improve the tubules circumference and interstitial area significantly (P<0.05). The mechanism of spermatogenic cells repairs suspected due to various bioactive molecules that secreted by hWJ-MSCs which can affect the surrounding cells.

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Reviewer

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Contact Name
Fika Kharisyanti

Contact Email
fikakharisyanti@gmail.com

Phone
+6282232687366

Journal Mail Official
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Editorial Address
Ruang Stem Cell, Gedung Lembaga Penyakit Tropis Lantai 2, Kampus C Universitas Airlangga

Location
Kota surabaya,

Jawa timur

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Fika Kharisyanti

Contact Email fikakharisyanti@gmail.com

Phone +6282232687366

Journal Mail Official -

Editorial Address Ruang Stem Cell, Gedung Lembaga Penyakit Tropis Lantai 2, Kampus C Universitas Airlangga

Location Kota surabaya, Jawa timur INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Fika Kharisyanti

Contact Email
fikakharisyanti@gmail.com

Phone
+6282232687366

Journal Mail Official
-

Editorial Address
Ruang Stem Cell, Gedung Lembaga Penyakit Tropis Lantai 2, Kampus C Universitas Airlangga

Location
Kota surabaya,

Jawa timur

INDONESIA